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KMID : 0861120110150030127
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Korean Journal of Oriental Preventive Medicine
2011 Volume.15 No. 3 p.127 ~ p.140
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The antitumor activities of Acanthopanax senticosus Harms(ASH) in human gastric cancer AGS cell lines
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Lee Sun-Dong
Ko Seong-Gyu
Shin Heon-Tae
Shin Yong-Cheol
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Abstract
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Objectives: The research was conducted to confirm the effect of Acanthopanax senticosus harms(ASH)
on the anti-tumor activities in AGS human gastric cancer cells.
Methods: To examine the potential anti-tumor effect of ASH, we performed many experiments. After
processing AGS cancer cells with varying concentrations 80% ethanol ASH extract, analyses by MTT,
flow cytometer(FACS) and western blot were used.
Results: AGS cancer cells showed decreased cell proliferation and increased contents of S phase when
treated with ASH. Moreover, the Western blot experiment showed that ASH affected S phase cell
cycle-related molecules(Cyclin A, p21 and p16) in AGS cells. ASH also inhibited EGFR-STAT3 pathway
in AGS human gastric cancer cells.
Conclusion: Based on these results, we observed that ASH arrested the cell cycle at S phase and inhibited
the phosphorylation of EGFR and STAT3 proteins which reduce the cell cycle and the manifestation of
the genes that are related to inhibiting cell growth in AGS cells. It can be concluded that ASH can be
used in developing medicine for gastric cancer.
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KEYWORD
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Acanthopanax senticosus Harms(ASH), Gastric cancer, cell cycle, apoptosis, EGFR-STAT3 pathway
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